how can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?
Swapnil Patil
Carbonate-bicarbonate buffer stock (pH 9.2)
1. Prepare a 0.2-M solution of anhydrous sodium carbonate (2.2 g/100 mL).
2. Prepare a 0.2-M solution of sodium bicarbonate (1.68 g/100 mL).
3. Combine 4 mL of carbonate solution from Step 1 and 46 mL of bicarbonate solution from Step 2.
4. Bring to 200 mL with H2O. Final pH will be 9.2.
Use this stock to make 0.05M Carbonate buffer
Ref-Cold Spring Harbor Protocol
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