How can we stain d tissue section by mallory triple stain?

How can we stain d tissue section by mallory triple stain? If any one has the protocol of mallory triple stain then please help.

08 September 2018 7,550 1 View

Can we use phosphate buffer pH 8 for different parameters of MDA , reduced gultathione ,Catalase , SOD?

Can we use phosphate buffer pH 8 for tissue homogenate formation for different parameters of MDA ,reduced gultathione ,Catalase , SOD?

06 July 2017 3,532 13 View

Anyone has the standardized protocol for prepration of dose for rat by powdered curcumin ?

04 May 2017 1,874 2 View

How to calculate the specific activity of MDA by wilbur method ?

how to calculate the specific activity of MDA by wilbur method ?

09 October 2016 6,234 1 View

How to calculate the specific activity of GPx by rotruck method ?

how to calculate the specific activity of GPx by rotruck method ?

09 October 2016 2,003 2 View

How can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?

how can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?

08 September 2016 3,234 1 View

What is the differnce between competitive RT- PCR and real time PCR?

what is the differnce between competitive RT- PCR and real time PCR?

07 August 2016 4,190 0 View

Can anyone give the protocol of giemsa satining of tissue ?

can anyone give the protocol of giemsa satining of tissue ?

07 August 2016 6,001 5 View

Can anyone give the protocol of giemsa satining of tissue after potasium dichromate fixation?

Can anyone give the protocol of giemsa satining of tissue after potasium dichromate fixation?

07 August 2016 4,045 0 View

How to identify chromosomal abbreations in bone marrow of mice ?

How to identify chromosomal abbreations in bone marrow of mice?

07 August 2016 2,692 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View