Hello
Look at link here , will help you
Good Luck
http://www.ihcworld.com/_protocols/special_stains/giemsa_ellis.htm
http://ocw.tufts.edu/data/15/342518.pdf
http://www.newcomersupply.com/documents/staining/procedures/Wright%20Stain%20Modified.1421.pdf
https://www.researchgate.net/search.Search.html?type=publication&query=Giemsa
https://www.researchgate.net/search.Search.html?type=question&query=Giemsa
It would be nice if we got any information which material/tissue should be stained after which specimen preparation....(fixation, Dehydration....) and yes and naturally: there are a lot of Giemsa - (Romanowski-stain) modifications [sometimes also in conjunction with other dyes, differing/different solutes, varied pH, etc. etc.) out in the wild [e.g. long - short staining, diverse material like blood, bacteria, smears, parasites, sections] looking forward to be found by e.g. http://Scholar.Google.com *) search. But usually one would prefer , etc (which most often can be found in (also institutional and hence specialized) libraries (:-)) , or - at least what Dr. Saleh in the previous post has recommended to look into. Best of luck.
Note edited: search for < Giemsa stain recipe OR method > yields approx. 94,300 results, < Giemsa stain recipe OR method OR Romanowski > approx. 93,600 results. One of them is linking to the Abstract of:
The Giemsa Stain: Its History and Applications, by Juan José Barcia (2007); http://ijs.sagepub.com/content/15/3/292.short
Dear Manisha, You have received links to useful sites above and you can learn a lot from papers, especially when authors describe using similar tissue/fixation/purpose of staining (parasites) and if there are good microphotographs. The websites such as IHCworld list descriptions of methods and some practical pointers. Dr Wolfgang Muss is right that having such a vast amount of information (staggering 94,600 results!) one tends to trust histology manuals. From the 'newer' manuals - I like to check information in 'Theory and Practice of Histological Techniques" edited by JD Bancroft and M Gamble - have added a Word file of my Lab Method folder with Giemsa method for tissue taken from the 6th edition of this manual. There is also a 7th edition (2015).
NB. The Giemsa stain history and applications link to JJ Barcia paper mentioned by Wolfgang is worth reading (and quoting, especially if it is a project you are working on and not a paper with limited number of references). A very important point made by the author of this paper is (verbatim quote):
1."The most important drawback of the Giemsa stainis that it is quick vanishing, especially if it is exposedto light; therefore, the slides are not permanent and
cannot be reviewed after a variable period, depending on the kind of Giemsa stock solution employed".
2. "As far as the technical aspects are concerned, the preparation of adequate slides is of supreme importance for microscopic analysis. It is not uncommon
to observe overly thick sections that are excessively stained and/or poorly differentiated and that are thus unacceptable for observation".
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