How to identify chromosomal abbreations in bone marrow of mice?
How can we stain d tissue section by mallory triple stain? If any one has the protocol of mallory triple stain then please help.
08 September 2018 7,678 1 View
Can we use phosphate buffer pH 8 for tissue homogenate formation for different parameters of MDA ,reduced gultathione ,Catalase , SOD?
06 July 2017 3,686 13 View
Anyone has the standardized protocol for prepration of oral dose for rat by powdered curcumin ?
04 May 2017 2,014 2 View
how can we prepare homogente from tissue for total cholestrol estimation?
02 March 2017 1,099 1 View
how to calculate the specific activity of MDA by wilbur method ?
09 October 2016 6,445 1 View
how to calculate the specific activity of GPx by rotruck method ?
09 October 2016 2,217 2 View
how can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?
08 September 2016 3,458 1 View
what is the differnce between competitive RT- PCR and real time PCR?
07 August 2016 4,340 0 View
can anyone give the protocol of giemsa satining of tissue ?
07 August 2016 6,126 5 View
Anyone has method of stadtmen for cholestrol detrmintaion ?
07 August 2016 8,262 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
