How can we stain d tissue section by mallory triple stain?

How can we stain d tissue section by mallory triple stain? If any one has the protocol of mallory triple stain then please help.

08 September 2018 7,550 1 View

Can we use phosphate buffer pH 8 for different parameters of MDA , reduced gultathione ,Catalase , SOD?

Can we use phosphate buffer pH 8 for tissue homogenate formation for different parameters of MDA ,reduced gultathione ,Catalase , SOD?

06 July 2017 3,532 13 View

Anyone has the standardized protocol for prepration of dose for rat by powdered curcumin ?

04 May 2017 1,874 2 View

How can we prepare homogente from tissue for total cholestrol estimation?

how can we prepare homogente from tissue for total cholestrol estimation?

02 March 2017 982 1 View

How to calculate the specific activity of MDA by wilbur method ?

how to calculate the specific activity of MDA by wilbur method ?

09 October 2016 6,234 1 View

How to calculate the specific activity of GPx by rotruck method ?

how to calculate the specific activity of GPx by rotruck method ?

09 October 2016 2,003 2 View

How can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?

how can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?

08 September 2016 3,234 1 View

What is the differnce between competitive RT- PCR and real time PCR?

what is the differnce between competitive RT- PCR and real time PCR?

07 August 2016 4,190 0 View

Can anyone give the protocol of giemsa satining of tissue ?

can anyone give the protocol of giemsa satining of tissue ?

07 August 2016 6,001 5 View

Can anyone give the protocol of giemsa satining of tissue after potasium dichromate fixation?

Can anyone give the protocol of giemsa satining of tissue after potasium dichromate fixation?

07 August 2016 4,045 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View