Can we use phosphate buffer pH 8 for different parameters of MDA , reduced gultathione ,Catalase , SOD?

Can we use phosphate buffer pH 8 for tissue homogenate formation for different parameters of MDA ,reduced gultathione ,Catalase , SOD?

06 July 2017 3,532 13 View

Anyone has the standardized protocol for prepration of dose for rat by powdered curcumin ?

04 May 2017 1,874 2 View

How can we prepare homogente from tissue for total cholestrol estimation?

how can we prepare homogente from tissue for total cholestrol estimation?

02 March 2017 982 1 View

How to calculate the specific activity of MDA by wilbur method ?

how to calculate the specific activity of MDA by wilbur method ?

09 October 2016 6,234 1 View

How to calculate the specific activity of GPx by rotruck method ?

how to calculate the specific activity of GPx by rotruck method ?

09 October 2016 2,003 2 View

How can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?

how can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?

08 September 2016 3,234 1 View

What is the differnce between competitive RT- PCR and real time PCR?

what is the differnce between competitive RT- PCR and real time PCR?

07 August 2016 4,190 0 View

Can anyone give the protocol of giemsa satining of tissue ?

can anyone give the protocol of giemsa satining of tissue ?

07 August 2016 6,001 5 View

Can anyone give the protocol of giemsa satining of tissue after potasium dichromate fixation?

Can anyone give the protocol of giemsa satining of tissue after potasium dichromate fixation?

07 August 2016 4,045 0 View

How to identify chromosomal abbreations in bone marrow of mice ?

How to identify chromosomal abbreations in bone marrow of mice?

07 August 2016 2,692 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View