Swapnil Patil

5 Questions 21 Answers 0 Followers

Questions related from Swapnil Patil

What type of PC calibrator should be used and does the Cutoff determination should be based on Positive control calibrator?

I'm trying to develop a chemi-luminescence based immunoassay for HIV antibody detection. For the purpose I'm using negative as well as positive control as a quality control. Currently, I'm using...

12 December 2019 3,179 2 View

How can the stability of Biotin conjugated/ HRP conjugated antibodies and antigen in ELISA/ Chemiluminescence assay can be achieved?

I'm trying to standardize a buffer in which my biotin conjugated and HRP conjugated molecules (antibodies and antigen) stay stable for longer time. My assay for detection of HIV works well with no...

06 June 2019 7,234 3 View

What should I use for cDNA synthesis?

I want to do cDNA synthesis and would like your suggestion regarding what to use, Oligo dT or Random primer. I would like if you also give me the reference for the same.

11 November 2012 10,002 25 View

What is Terahertz dance, can anyone please explain about it?

The only thing I know, about it is, is related to proteins.

09 September 2011 4,475 8 View

How to determine the stability of Phosphate buffered saline (PBS)?

PBS is commonly used for many purposed in the labs, most of the time it is freshly prepared but what in case it is made and kept for longer time. How long can it be stored and how to access its...

01 January 1970 9,573 4 View