what is the differnce between competitive RT- PCR and real time PCR?
How can we stain d tissue section by mallory triple stain? If any one has the protocol of mallory triple stain then please help.
08 September 2018 7,550 1 View
Can we use phosphate buffer pH 8 for tissue homogenate formation for different parameters of MDA ,reduced gultathione ,Catalase , SOD?
06 July 2017 3,532 13 View
04 May 2017 1,874 2 View
how can we prepare homogente from tissue for total cholestrol estimation?
02 March 2017 982 1 View
how to calculate the specific activity of MDA by wilbur method ?
09 October 2016 6,234 1 View
how to calculate the specific activity of GPx by rotruck method ?
09 October 2016 2,003 2 View
how can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?
08 September 2016 3,234 1 View
can anyone give the protocol of giemsa satining of tissue ?
07 August 2016 6,001 5 View
Can anyone give the protocol of giemsa satining of tissue after potasium dichromate fixation?
07 August 2016 4,045 0 View
How to identify chromosomal abbreations in bone marrow of mice?
07 August 2016 2,692 0 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
