Gene synthesis - pros and cons of codon optimization?

Dear all, I am synthesizing a nematode gene for reexpression in human cells and functional characterization (not for protein expression/purification). What are the pros and cons of codon...

04 November 2019 2,399 2 View

How to estimate regression to find which firm-specific characteristics explain cross-industry heterogeneity in the firm returns?

I would kindly like to ask for guidance regarding my question below. To illustrate my question: I have two industries: Industry 1 and Industry 2. Both industries contain a number of firms that...

20 May 2019 4,907 1 View

Is there a method to make a serum working on paraffin embedded tissue?

We have raised a rabbit antiserum against a protein coupled peptide. This peptide has been reported to be antigenic and some papers have been published with a commercial preparation raised in the...

22 January 2017 5,681 4 View

Can anyone recommend antibodies for histochemistry of rat macrophages and B-cells?

We try to detect macrophages and B-cells in rat bone-tissue. However, we get cross reaction when using mouse monoclonals. Has anybody experienced similar problems and developed a solution?

15 December 2016 2,972 4 View

Which exon should I target for CRISPR-Cas9 system?

Is there any rule/preference which exon should I target to knock out the GOI by CRISPR-Cas9 system?

13 November 2016 4,728 5 View

Who has experience with activity assays for FOLH1?

We have observed a massive transcriptional regulation of FOLH1 in cell cultures. This protein is also known as Prostate-Specific Membrane Antigen or Glutamate Carboxypeptidase II. However we were...

25 October 2016 4,658 2 View

Does anybody know a simplified methodology or equation to estimate the temperature of a solar collector/absorber?

Does anybody know a simplified methodology or equation to estimate the temperature of a solar collector/absorber? I want to calculate the energy performance and the energy yield. I have Data for a...

17 March 2016 4,911 5 View

Does my ligated plasmid ready for sequencing and is the purity OK?

I performed a ligation of 1.5 kb insert into a 4.2 kb vector and I got colonies on my kanamycin plate. I picked up 8 colonies and made a miniprep of them. 2 out of the 8 samples contained the...

15 February 2016 860 3 View

How do we solve our problem with mouse hippocampal cell cultures on 35 mm petri dishes?

Hi there,We have some problems with (E17-18) mouse hippocampal cell cultures. After sacrificing mice (E17-18), primary neurons were cultured (400,000 cells per on the 35 mm petri dishes). For...

08 February 2015 7,460 6 View

Mammalian expression vector for isolation of native protein complexes?

Hi, Can anyone recommend a specific mammalian expression vector that I could use to tag my protein of interest and that would allow me to purify any associated complexes for MS analysis? I am...

13 July 2014 5,410 10 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

Where would? can? positive charge(vacancy) be when grounded?

Hello I plasma(H2N2) treated Al2O3 plate (edge is connected to ground) and wondered if oxygen vacancy migration on the plate can occurs as electrons escape through edge. Or whatever positive...

15 February 2021 8,118 1 View

ZnS oxidizes to ZnO!!why??

when I put my ZnS solution in the chemical bath, the ZnS oxidizes so there are ZnO peaks appearing because of the reaction with the oxygen in the air, despite the fact that I close the beaker...

14 February 2021 5,778 9 View

Aspen Plus Error: Unknown or undefined bead number?

Hello, in my Aspen Plus Simulation (see attachment) there are two heat exchangers. One HEX1 (oil+gas) ist fed by hot gas which ist coming out of a burner. The other HEX2 (gas+gas) shall work as a...

14 February 2021 8,096 2 View

About XRD details of pristine and doped RGO?

Hi Can anyone please help me know the XRD details of reduced graphene oxide, Nitrogen doped reduced graphene oxide, Sulphur doped reduced graphene oxide, Nitrogen and Sulphur co-doped reduced...

08 February 2021 3,033 3 View

How to prepare OH- terminated Si Wafer?

Hi, Folks. I am working on the ALD project to deposit Al2O3 on the Si wafer from TMA and Water. I have struggled with the preparation of OH- terminated Si wafer. I have both Si(1 0 0) and...

08 February 2021 8,792 2 View

Is it possible to have a diffusion of oxygen from the glass substrate (SiO2) into the absorbent layer during annealing process?

02 February 2021 382 7 View

What is the best visualisation solvent for xylooligosaccharides using TLC?

I am interested in using TLC to detect non-linear xylooligosaccharides. What would be the best visualisation solvent? Would aniline phthalate solution be suitable? I have also seen people...

02 February 2021 7,748 3 View

How to do ORR test with pine(Which means RDE or RRDE electrode)?

I want to do LSV ,CV test of ORR(Oxygen reduction reaction). But our laboratory don't have the RDE and RRDE electode. And I was wondering if I can do the tests by magnetic stiring or mechanical...

14 January 2021 1,869 2 View