Anyone has the standardized protocol for prepration of dose for rat by powdered curcumin ?

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How can we stain d tissue section by mallory triple stain?

How can we stain d tissue section by mallory triple stain? If any one has the protocol of mallory triple stain then please help.

08 September 2018 7,678 1 View

Can we use phosphate buffer pH 8 for different parameters of MDA , reduced gultathione ,Catalase , SOD?

Can we use phosphate buffer pH 8 for tissue homogenate formation for different parameters of MDA ,reduced gultathione ,Catalase , SOD?

06 July 2017 3,686 13 View

How can we prepare homogente from tissue for total cholestrol estimation?

how can we prepare homogente from tissue for total cholestrol estimation?

02 March 2017 1,099 1 View

How to calculate the specific activity of MDA by wilbur method ?

how to calculate the specific activity of MDA by wilbur method ?

09 October 2016 6,445 1 View

How to calculate the specific activity of GPx by rotruck method ?

how to calculate the specific activity of GPx by rotruck method ?

09 October 2016 2,217 2 View

How can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?

how can we prepare sodium carbonate buffer of 0.05 M from sodium carbonate and soidium bicarbonate?

08 September 2016 3,458 1 View

What is the differnce between competitive RT- PCR and real time PCR?

what is the differnce between competitive RT- PCR and real time PCR?

07 August 2016 4,340 0 View

Can anyone give the protocol of giemsa satining of tissue ?

can anyone give the protocol of giemsa satining of tissue ?

07 August 2016 6,126 5 View

Anyone has method of stadtmen for cholestrol detrmintaion ?

07 August 2016 8,262 1 View

How to identify chromosomal abbreations in bone marrow of mice ?

How to identify chromosomal abbreations in bone marrow of mice?

07 August 2016 2,814 0 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

How can I find access to heavy metal reference doses from the EPA and WHO websites?

Reference dose and Maximum acceptable concentrations HMs

03 August 2024 8,230 4 View

What can be the minimum number of animals (rats) to be used for a pilot/preliminary study on intradermal drug delivery?

Dear All I would like to know the minimum number of animals (rats) to be studied for a pilot/preliminary research on intradermal drug delivery. Can I proceed with 1 animal per group for a pilot work?

02 August 2024 4,570 1 View

How to convert g/kg Humic acid dose to kg/ha?

I used humic acid at 0.044 g/kg soil in my pot experiment. But finally, I have to recommend kg/ha. Each pot's soil weight was 11 kg. What is the solution?

02 August 2024 7,186 6 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View