I have an aliquot of M13K07 helper phage that was given to our lab by another lab. The titer says 2 * 10^13 CFU/mL, however, I want to verify this titer is correct. I infected ER2738 cells with the helper phage for about 45 min and then plated a dilution of this on Kanamycin plates, which gave me a result of 7.56*10^11 CFU/mL. I was wondering if this procedure is correct, or if the issue of superinfection will give me a false titer. How can I be sure how much phage is in my aliquot? I am wondering because I want to make sure I am producing my phage (using a phagemid helper phage system) using a decent MOI.