I have an aliquot of M13K07 helper phage that was given to our lab by another lab.  The titer says 2 * 10^13 CFU/mL, however, I want to verify this titer is correct.  I infected ER2738 cells with the helper phage for about 45 min and then plated a dilution of this on Kanamycin plates, which gave me a result of 7.56*10^11 CFU/mL.  I was wondering if this procedure is correct, or if the issue of superinfection will give me a false titer.  How can I be sure how much phage is in my aliquot?  I am wondering because I want to make sure I am producing my phage (using a phagemid helper phage system) using a decent MOI.

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