Paige Spencer

4 Questions 9 Answers 0 Followers

Questions related from Paige Spencer

Will my phage titers be affected if I accidentally left them on the counter at room temperature overnight instead of stored in 4°C?

I produced phage and purified them using PEG precipitation, then resuspended them in PBS. I was doing phage titers and accidentally left them on the counter overnight, instead of storing at 4°C. ...

25 May 2016 8,528 3 View

How can I titer an aliquot M13K07 helper phage?

I have an aliquot of M13K07 helper phage that was given to our lab by another lab. The titer says 2 * 10^13 CFU/mL, however, I want to verify this titer is correct. I infected ER2738 cells with...

25 May 2016 892 2 View

How do I know if the bacterial strain I am using (ER2738) has an F' plasmid which encodes resistance to antibiotic?

I know the strain itself is, but does that imply that it is due to the F' plasmid?

14 January 2016 1,346 4 View

What are the best resources to learn about bacteriophage production?

I'm looking for literature that details optimal conditions for phage production.

12 January 2016 9,841 3 View