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Hi We were transfecting HEK-293 cells adapted to grow in suspension to produce recombinant proteins with excellent results. Cells were maintained in Freestyle, transfected with PEI/DNA also in...
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Higher affinities result in higher ELISA signals, of course. But now Im working with several mutated ligands and their receptor. The wt ligand has an affinity to the receptor around 20nM. Several...
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