After incubation of the TG1 cells with the helper phage, I spun down the culture and precipitated the supernatant with PEG/NaCl. After this, I spun it down again and resupended the pellet in PBS. This mixture was then again spun down at 11000 g. The resultant supernatant I obtained was quite viscous. What can be the possible reason for this? Are my phage clones aggregating? Can this affect the binding of the phage library to the coated antigen in the next round of panning?
One possible explanation is that a fraction of the TG1 host lysed and released genomic DNA into the supernatant. That could be an explanation for the viscosity.
Hey,
one explanation could be that you have a very high concentration of phage particles. So you are producing good amount of phage, which leads to a viscous solution.
Cheers,
Stephan
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