I need to eventually combine this solution with a .1M sodium acetate buffer with triton X. Eventually, this will be mixed with lysates and read for the presence of 4-MU fluorescence. I started out with trying to use chloroform but obviously this did not mix with the aqueous buffer. I wanted to use DMSO but I need a final concentration of .345mM and it's solubility in DMSO is only .1mg/mL. I tried heating the buffer to 70C and put in enough for .345mM (I found this in a publication) but this also didn't work. Does anyone have any ideas/experience?

Thank you!

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