How can I make 3.8 ml ethanol solution of DPPH radical (final concentration was 0.1 mM)?

More Abul Hossain's questions See All

Hi It will be better if I get answer about it?

How can I make BSA(20mg/10mL) for ELISA experiment?

05 June 2016 6,475 3 View

Hi,I need some information?

I need some information... 1) How many days prepared PBS can be used for ELISA experiment? Because I prepared PBS 25 days ago but after 25 days,if I do ELISA by this PBS,it will be any false for...

05 June 2016 7,678 4 View

What is the best way to wash microplate assay for ELISA experiment?

Dear brothers and friends What is the best way to wash microplate assay for ELISA experiment? how much time can I take to melt PnPP Substrate in buffer solution?

05 June 2016 3,739 1 View

What is the different between MAP and CAP?

In terms of food packaging.

02 March 2016 5,187 8 View

Could anyone suggest about 5% phenol preparation?

Though I know 5% phenol solution means 5 g phenol crystal for 100 mL distilled water, but, I am concerned about melting of phenolic crystal in water using heat. Could anyone suggest me how can I...

01 January 1970 7,005 5 View

How can I measure the impedance by using potentiostat?

I am working on the development of SWCNTs-based biosensor for rapid detection of bacteria. I would like to measure the interactions caused by the binding of antibody-antigen upon the biosensor...

01 January 1970 5,689 3 View

What is the protocol to make m-phosphoric acid reagent in glacial acetic acid ?

To determine Vit-C.

01 January 1970 1,836 4 View

How can I express ABTS result in percentage?

I want to express ABTS in %. I have extracted 5g powder sample in 50 ml water then diluted 100 times. For an example- ABTS (%)=100-[(O.D of sample/O.D. of control)*100] Here, O.D of...

01 January 1970 899 3 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How can I calculate IC50 in DPPH test by using graph pad prism software?

How can I calculate IC50 in the DPPH test using graph pad prism software? or suggest a manual method and other software to calculate the IC50 value.

06 August 2024 8,848 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View