I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a significant challenge: my extracts are highly colored, which interferes with the colorimetric readings inherent to the MTT assay. This interference could potentially lead to inaccurate results.
Could anyone recommend alternative methods for assessing cell toxicity that are less susceptible to interference from colored compounds?
Dear Dr. @Abolfazl Dashti,
Flow Cytometry is too much for my purpose and expensive. I wanna find the cytotoxicity of my extracts.
I used a resazurin assay to determine cytoxicity of various plant extracts on fibroblasts. After exposure, the media containing the plant extract is aspirated prior to addition of the resazurin reagent. I tried using MTT briefly, but even with rinsing the test solutions prior to MTT addition, there was still too much background from the extracts. Rinsing with DPBS did help a lot, however. Resazurin also reacts with the plant extracts if it is not removed prior to the viability assay, but far less than MTT.
Dear Melissa Jean Towler
Thank you for your valuable suggestions. I will incorporate them into my methodology and will share the results here once I've tested the modifications.
Best regards
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