Lalrinzuali Khawlhring

13 Questions 35 Answers 0 Followers

Questions related from Lalrinzuali Khawlhring

What is validation of data in HPLC?

In HPLC, what is the validation of data? How to do it?

11 November 2017 9,569 5 View

Can someone provide a recent reference for the estimation of Collagen (Woessner, 1961), DNA (Burton, 1956), Lipid peroxidation (Buege and Aust) etc?

hi, i need recent references for the estimation of Collagen which is similar to the protocol described by Woessner (1961), DNA similar protocol described by Burton, (1956), Lipid peroxidation...

09 September 2017 4,423 3 View

Is there any way to clean/recover cell lines contaminated with bacteria??

i have observed contamination on some of my cell lines, bacterial stain indicated its a coccus type of bacteria, i still need the cells so is there any way to get rid of them??

02 February 2016 6,349 4 View

What is the most important enzyme for human and why?

among the endogenous enzymes like, glutathione, superoxide, catalase and glutatione-s-transferase, what would be the most important for human ?? and what is the reason?? thank you

02 February 2016 4,340 6 View

What does it mean when treatment of tumor cell line using plant extract gives biphasic curve?

i have treated a tumor cell line using a plant extract and terminated the treatment at different times (eg: 1 hour, 2 hours, 4 hrs and so on) and estimated some enzyme activities and observed that...

02 February 2016 1,038 3 View

How exactly does bacterial contamination/ the use of antibiotics in media affect cells??

So i have heard that bacteria release toxins to the media and can retard cell growth, but i don't know how exactly it affect the cells and what changes can occur... is it by changing the pH of the...

02 February 2016 2,201 4 View

How to store hela cell homogenates?

i have 102 samples and i have to estimate protein, gsh, lipid peroxidation, gst, gshpx, catalase and sod..... it will take weeks to finish all these assays, so, after making cell homogenates in...

01 January 2016 3,564 2 View

Why plant extracts/drugs have more activity in one cell line as compared to other cell lines?

i have noticed that the in- vitro anticancer activities of my plant extract and an isolated compound differ on different cell lines....... what could be the reason behind?

12 December 2015 3,480 7 View

My MTT reading is fluctuating.. can someone help me?

i have performed MTT as per Mosmann T on HepG2 cell line using different concentrations of plant extracts (1-90 ug/ml). The absorbance of all the treated groups are reduced to about half as...

11 November 2015 9,842 10 View

Hi, is there any facility in india where I can send my plant extracts for in vitro anticancer activity tests?

i need mtt, clonogenic, comet assay etc.

07 July 2015 9,758 7 View

Can pure DMSO be used as a vehicle in mice?

I need to do anticancer activity and some biochemical parameters of plant extract in mice, my extracts dissolves well in pure DMSO. Is it safe to use pure DMSO as a vehicle if not, what percentage...

10 October 2013 10,098 12 View

Can someone tell me if i can run sephadex Lh 20 with chloroform and methanol as a solvent system?

My sample dissolves only on chloroform and methanol but neither on pure chloroform nor pure methanol.... so, would like to know if i can subject it to sephadex Lh-20 using chloroform and methanol...

08 August 2013 3,968 10 View

Is there any simple way for isolation of alkaloid from a column fraction?

06 June 2012 3,930 4 View