Dear Sir. Concerning your issue about the preparation of 5% phenol. I think the following below links may help you in your analysis:

https://stockingerlab.osu.edu/sites/stockinger/files/imce/PDFs/Protocols/PhenolPrep.pdf https://link.springer.com/content/pdf/bbm%3A978-94-010-9139-8%2F1.pdf

http://www.mrc-lmb.cam.ac.uk/ms/methods/phenol.html

Thanks

Dear Abdul,

You may use certain buffers such as TrisCl or TE buffer.

You may adopt your procedure from here: http://www.mrc-lmb.cam.ac.uk/ms/methods/phenol.html

Best,

Ariz

http://www.scielo.cl/pdf/maderas/v16n2/aop1314.pdf

https://www.atsdr.cdc.gov/toxprofiles/tp115.pdf

Phenol is moderately soluble in water - about 8 g of phenol will dissolve in 100 mL water. So, take 5 gm phenol crystals and add water to 100 mL.

Here,

this might help.

Poster Increasing water solubility of Quercetin by increasing the temperature