How to find orthotropic material properties?

I am doing an impact analysis of bullet on a plate. But for that simulation, I require orthotropic properties of materials like PPS and PEEK which are Unidirectional type. How to find them?...

16 February 2021 8,775 2 View

Error While inserting Properties in ANSYS Data?

I want to do analysis in the ANSYS ACP module. For the same, I am trying to insert Properties of material in which I need to add Isotropic Elasticity ,orthotropic stress limit and Ply Type. But If...

30 January 2021 4,438 3 View

Is it possible to analyse the High velocity bullet impact on plate from 10 meter distance? In which software it ANSYS or ABAQUS or LSDYNA?

Hello all, Im Ajinkya. Doing analysis of High velocity bullet on plate made of 3 different materials from 10m distance. In which software it will be good for analysis ANSYS or LSDYNA or ABAQUS?

04 January 2021 9,462 4 View

Is there any FDA approved polymer or IIG listed polymer which is easily soluble in sesame or castor oil ?

I am in search of a polymer which has a solubility in oil.

25 December 2020 9,481 2 View

Reasons for hysys to crash while carrying out optimization using MATLAB ?

I am carrying out simulation based optimization by connecting MATLAB and Hysys using Activex. Hysyslib is used to connect hysys and MATLAB I am deploying genetic algorithm to carry out...

24 April 2020 1,899 3 View

Quantifying IHC between treatment groups?

I am looking for input on how to quantify the amount of signal from lung tissue sections stained with anti- alpha SM actin. Is there a program out there that I can upload images of my tissue and...

10 March 2020 4,044 3 View

How can I model a 2-D(axial and radial variation of variables) dynamic heterogeneous reactor model equations (PDE) ?

I have 2-D dynamic gas-solid reaction model, with variables( temperature, pressure, velocity) varying in radial and axial directions. The fluid and solid phase are not in equilibrium and momentum...

17 June 2019 416 1 View

How to verify whether MANT-GDP bound to Ras protein or not?

I am doing nucleotide exchange assay using Tecan spark 10 M machine.I have loaded KRas with MANT-GDP and used 1uM of the same and the relative Fluorescence units is around 32000. But the...

30 March 2019 5,852 4 View

How to prepare E.Coli cell lysate as I want to use it as negative control during SDS PAGE alongside my protein lysate ?

I tried using E.coli BL21 cells -grew them in 2TY media for about 2 hours ,centrifuged and lysed the cells using lysozyme and DNAse ? But i only got a single band at the bottom of my SDS Gel .

18 August 2017 6,260 3 View

What is a suitable solvent to dissolve diphenyl-diselenide?

Thanks in advance for your replies.

14 April 2015 2,572 5 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will it be possible to get IC:50 if we target cancer cell line with antibody drug conjugate (ADC) that have 50% less antigen positive cells ?

Is it possible to attain IC50 if my target cells have less than 50% antigen expression ? For example: Cancer cell line XXX is showing 35% of expression of cell surface antigen YYYY, if i...

04 February 2021 2,711 3 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

Why do the absorbance values of different concentrations of ascorbic acid solutions mixed with DPPH free radicals stay constant?

26 January 2021 458 14 View

IC50 value from this graph?

01 January 2021 6,848 4 View

I am performing enzyme inhibition assay and the substrate is fluorogenic & fluorescence was measured. Now I want to calculate Vo and Vi. How can I ??

These are the per minute fluorescence observed. Please guide me how can I determine Vi, Vo and IC50 ? By slope determination or any other method? Control: 28.11 40.30 40.78 37.61...

11 December 2020 1,037 4 View

How to determine non-cytotoxic drug concentration using MTT assay?

Many literature showed that how to determine IC50 drug concentration using MTT assay. But I want to know how to determine non-cytotoxic concentration of a particular drug with maximum its...

31 October 2020 2,012 3 View

Is rotary evaporator a must before going through TPC, TFC and DPPH assay?

I am doing an optimization of ginger extract using doe software, firstly I extract the ground ginger with 20ml solvents of 60%, 70% and 80% ethanol using the ultrasound water bath in different...

23 October 2020 3,268 10 View

How do I find the IC50 and best drug treatment time for anticancer drug?

I want to find the IC50 of a drug on a cancer cell line. I'm not sure how long the treatment time should be for in vitro experiment. Is there an assay/protocol I can follow to determine the IC50...

15 October 2020 8,678 6 View

How to calculate IC50 of plant extracts dissolved in DMSO?

Greeting! Currently I am working with my plant extracts with different concentration on DPPH assay and anti-tyrosinase assay. All of my plant extracts with different concentration are dissolved...

02 October 2020 3,185 3 View