I need some information...
1) How many days prepared PBS can be used for ELISA experiment? Because I prepared PBS 25 days ago but after 25 days,if I do ELISA by this PBS,it will be any false for experiment or not?
2) After dilution any chemical,how many days can it be used for experiment?
PBS can be used as long as it doesnt have any visible contamination. 25 days is not a long time for PBS. Dont worry about that. If you have doubts, just re-check the pH.
The possible storage time depends on the chemical, of course. I dont know which other chemicals/solutions are you talking about. For instance, many substrate solutions are freshly prepared before using them in ELISA, unless they are commercial ready-to-use solutions.
I have seen people using it in a range between 1% and 5%. When I use BSA as blocking agent, I normally pprepare it at 4% in PBS. But most of the times, I prefer to use 4% skim powder milk instead.
very good response by Rojas. Details of Guidelines and protocols, see it
https://tools.thermofisher.com/content/sfs/brochures/TR0065-ELISA-guide.pdf
- Badges
- Science method
- Similar topics
- Medicine
- Immunology
- Immunology Techniques
- Immunoassay
- ELISA