Immunological effect of matrigel on mouse immune system ??

Matrigel is very excellent material for tumor engraftments. We know its positive effects only. Does anyone know about its immunological effect on mouse immune system ? Thanks and regards Anuj

03 April 2019 5,260 0 View

Can anyone share a reference showing slpeen weight of DBA/2 mice ( Naive ) 10-12 week old ?

Hi, I am working with DBA/2 mice and I want to know spleen weight of naive and nulliparour 10-12 week old female DBA/ mice Please help me Regards Anuj

10 November 2017 1,065 0 View

4T1 cell line is contaminated with Mycoplasm ?

I have 4T1 cell line and it is contaminated with Mycoplasma .I passed it through nude mice subcutaneous injection and cultutered tumor cell and tested for mycoplasma presence and found it's...

10 November 2016 9,897 6 View

How much possibility is there that mycoplasma will infect murine tumor cell line ?

If anyone have any adea about the corelatiob between mycoplasma and murine tumor cell line like B16F10, CT26 and 4T1 Anuj

07 August 2016 974 7 View

Hi friends, I want to know about any recent research on topic " How to remove mycoplasma from mycoplasma contaminated cells injected in Nude mice ?

Please provide your inputs on this topic

07 August 2016 973 0 View

Is any link between medium volume, cell growth and gaseous exchange during the culture of cancer cell line ?????

04 May 2016 7,436 7 View

How can I identify graft rejection in female nude mice?

31 December 2015 7,748 1 View

How can I harvest tumor cells from matrigel coated flask?

07 August 2015 6,315 5 View

Could anyone tell me that if I inject evans blue dye (20mg/kg-5mL/kg) into lung of mice, will the mice survive or not for at least one week?

Thanks in advance for your replies.

04 May 2015 4,239 4 View

Can any one help me the coordinates to inject the cells for orthotopic GBM Model development using U87 cell line?

Please can you tell me the coordinates ( x, y and z from bregma ) to inject the cells for orthotopic GBM Model development using U87 cell line?

11 December 2014 7,222 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View