Please provide your inputs on this topic
Matrigel is very excellent material for tumor engraftments. We know its positive effects only. Does anyone know about its immunological effect on mouse immune system ? Thanks and regards Anuj
03 April 2019 5,380 0 View
Hi, I am working with DBA/2 mice and I want to know spleen weight of naive and nulliparour 10-12 week old female DBA/ mice Please help me Regards Anuj
10 November 2017 1,166 0 View
I have 4T1 cell line and it is contaminated with Mycoplasma .I passed it through nude mice subcutaneous injection and cultutered tumor cell and tested for mycoplasma presence and found it's...
10 November 2016 10,008 6 View
I want to develop LTPA cell line tumor model in mouse and tried Balb/c and C57BL/6 mice but I did not get any tumor If anyone have any idea please help me to get the model Regards Anuj
10 November 2016 8,857 0 View
If anyone have any adea about the corelatiob between mycoplasma and murine tumor cell line like B16F10, CT26 and 4T1 Anuj
07 August 2016 1,079 7 View
Currently I am dealing with Calu-6 cell line . I want to keep the cells in T225cm2 flask with 2.8 million/T225cm2 with medium volume of 20mL/flask. I want to know what will be the rate of Gaseous...
04 May 2016 7,527 7 View
Recently I injected a cell line in female nude mice and after 7-8 days of injection , I found redness on skin of most of the mouse and after redness, whole skin of the animal have covered with...
31 December 2015 7,852 1 View
I am trying to harvest cell from matrigel coated flask plaese guide me I have tried trypsin but didn't work . I don't have matrigel dissociation medium
07 August 2015 6,448 5 View
Thanks in advance for your replies.
04 May 2015 4,362 4 View
Please can you tell me the coordinates ( x, y and z from bregma ) to inject the cells for orthotopic GBM Model development using U87 cell line?
11 December 2014 7,354 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/
08 August 2024 4,585 1 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View