Immunological effect of matrigel on mouse immune system ??

Matrigel is very excellent material for tumor engraftments. We know its positive effects only. Does anyone know about its immunological effect on mouse immune system ? Thanks and regards Anuj

03 April 2019 5,260 0 View

4T1 cell line is contaminated with Mycoplasm ?

I have 4T1 cell line and it is contaminated with Mycoplasma .I passed it through nude mice subcutaneous injection and cultutered tumor cell and tested for mycoplasma presence and found it's...

10 November 2016 9,897 6 View

I want to develop LTPA cell line tumor model?

10 November 2016 8,724 0 View

How much possibility is there that mycoplasma will infect murine tumor cell line ?

If anyone have any adea about the corelatiob between mycoplasma and murine tumor cell line like B16F10, CT26 and 4T1 Anuj

07 August 2016 974 7 View

Hi friends, I want to know about any recent research on topic " How to remove mycoplasma from mycoplasma contaminated cells injected in Nude mice ?

Please provide your inputs on this topic

07 August 2016 973 0 View

Is any link between medium volume, cell growth and gaseous exchange during the culture of cancer cell line ?????

04 May 2016 7,436 7 View

How can I identify graft rejection in female nude mice?

31 December 2015 7,748 1 View

How can I harvest tumor cells from matrigel coated flask?

07 August 2015 6,315 5 View

Could anyone tell me that if I inject evans blue dye (20mg/kg-5mL/kg) into lung of mice, will the mice survive or not for at least one week?

Thanks in advance for your replies.

04 May 2015 4,239 4 View

Can any one help me the coordinates to inject the cells for orthotopic GBM Model development using U87 cell line?

Please can you tell me the coordinates ( x, y and z from bregma ) to inject the cells for orthotopic GBM Model development using U87 cell line?

11 December 2014 7,222 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

22 February 2021 3,891 2 View