Anuj Singh

15 Questions 19 Answers 0 Followers

Questions related from Anuj Singh

Immunological effect of matrigel on mouse immune system ??

Matrigel is very excellent material for tumor engraftments. We know its positive effects only. Does anyone know about its immunological effect on mouse immune system ? Thanks and regards Anuj

04 April 2019 5,323 0 View

Can anyone share a reference showing slpeen weight of DBA/2 mice ( Naive ) 10-12 week old ?

Hi, I am working with DBA/2 mice and I want to know spleen weight of naive and nulliparour 10-12 week old female DBA/ mice Please help me Regards Anuj

11 November 2017 1,119 0 View

I want to develop LTPA cell line tumor model?

I want to develop LTPA cell line tumor model in mouse and tried Balb/c and C57BL/6 mice but I did not get any tumor If anyone have any idea please help me to get the model Regards Anuj

11 November 2016 8,802 0 View

4T1 cell line is contaminated with Mycoplasm ?

I have 4T1 cell line and it is contaminated with Mycoplasma .I passed it through nude mice subcutaneous injection and cultutered tumor cell and tested for mycoplasma presence and found it's...

11 November 2016 9,955 6 View

How much possibility is there that mycoplasma will infect murine tumor cell line ?

If anyone have any adea about the corelatiob between mycoplasma and murine tumor cell line like B16F10, CT26 and 4T1 Anuj

08 August 2016 1,022 7 View

Hi friends, I want to know about any recent research on topic " How to remove mycoplasma from mycoplasma contaminated cells injected in Nude mice ?

Please provide your inputs on this topic

08 August 2016 1,028 0 View

Is any link between medium volume, cell growth and gaseous exchange during the culture of cancer cell line ?????

Currently I am dealing with Calu-6 cell line . I want to keep the cells in T225cm2 flask with 2.8 million/T225cm2 with medium volume of 20mL/flask. I want to know what will be the rate of Gaseous...

05 May 2016 7,488 7 View

How can I identify graft rejection in female nude mice?

Recently I injected a cell line in female nude mice and after 7-8 days of injection , I found redness on skin of most of the mouse and after redness, whole skin of the animal have covered with...

01 January 2016 7,798 1 View

How can I harvest tumor cells from matrigel coated flask?

I am trying to harvest cell from matrigel coated flask plaese guide me I have tried trypsin but didn't work . I don't have matrigel dissociation medium

08 August 2015 6,388 5 View

Could anyone tell me that if I inject evans blue dye (20mg/kg-5mL/kg) into lung of mice, will the mice survive or not for at least one week?

Thanks in advance for your replies.

05 May 2015 4,305 4 View

How do I develop a orthotopic GBM model using the U87 cell line?

Please can any one help me to get the coordinates ( x, y and z from bregma ) to inject the cells for orthotopic GBM Model development using U87 cell line?

12 December 2014 1,752 2 View

Can any one help me the coordinates to inject the cells for orthotopic GBM Model development using U87 cell line?

Please can you tell me the coordinates ( x, y and z from bregma ) to inject the cells for orthotopic GBM Model development using U87 cell line?

12 December 2014 7,295 4 View

Can anyone suggest the right cell line to develop orthotopic Glioblastoma ?

I am going to develop orthotopic Glioblastoma mouse model. Can anyone tell me right cell line at right cell concentration and right injection volume. ? How much time it will take to develop the...

11 November 2014 1,331 5 View

Can anyone tell me exact injection site to develop orthotopic lung cancer in nude mice?

I am trying to develop orthotopic lung cancer model in nude mice. I want to inject the single cell suspension directly into lung parenchyma without surgery . After injection of cell into the lung...

10 October 2014 2,949 4 View

Does PC 3 (Prostate cancer, PTEN NULL) Cell line have a defect in RAD51?

01 January 2014 9,436 0 View