Please can you tell me the coordinates ( x, y and z from bregma ) to inject the cells for orthotopic GBM Model development using U87 cell line?
Apurva's answer is right for an orthotopic model. However drug delivery into the brain is particularly difficult, and we've done U87 xenografts (http://altogenlabs.com/xenograft-models/brain-cancer-xenograft/u87-xenograft-model/) ectopically with stable growth curves varying based on drug treatment. We do our injections into the hind leg, which is an easy spot for treatment regimens and the like.
The coordinates to inject cells using a stereotaxic apparatus is 2.5mm below (y) the bregma and inject at a depth (z) of 2mm using a hamilton syringe.
Good luck.
Apurva's answer is right for an orthotopic model. However drug delivery into the brain is particularly difficult, and we've done U87 xenografts (http://altogenlabs.com/xenograft-models/brain-cancer-xenograft/u87-xenograft-model/) ectopically with stable growth curves varying based on drug treatment. We do our injections into the hind leg, which is an easy spot for treatment regimens and the like.
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