I followed a method for the development of mammary tumours in spargue dawley rats by using DMBA. According to the article, after 15-20 weeks for the tumour growth could have shown. It is more than 30 weeks after the dose of DMBA and no tumour growth is observed. I could have the lesser average body weight of rats comparatively controls and vehicles after the treatment but no tumour induced till now. I want to induce mammary tumours in some of the rats transgenically or surgically or any other methods. Could anybody suggest something?
You could try using nude rats to engraft in human mammary cell lines. However, in my experience immunodeficient rats (nudes) are not as useful as the most immunodeficient mouse models, e.g. NSG mice. I dont know whether the sole purpose of your project is to develop a novel mammary model but you could try syngeneic models (rat 13762 cell line) such as that used by Neri et al. JNCI, VOL. 68, NO.3, MARCH 1982. Other alternatives you could try if you want to go down the transgenic route would be to use the p53 KO rats available from SAGE. Im pretty sure if you treated these with DMBA or ENU you will get cancer model....what cancer I dont know but might be interesting to try. Alternatively I believe you can get a SCID rat model also which might be useful for xenotransplantation. Good luck!
Dear Dr. Cormack,
Thanks for your suggestions.
Yes, you talked an interestingly to use p53 KO rats. The artical which you talk about is very interesting, also can help me much. I also request you for some such type of more articals, if you can suggest me..
Thanks..
Dear Dr Suman,
I'm very interested by this model of mammary tumors in rats induced by DMBA. Please, could you let me know if your experiment is based on the paper of De Coster et al. 1992 appearing in Cancer Res. 52:1240-1244.
Thank you in advance for your reply !
Thanks Dr. Benoit for your reply, I followed the paper of Lee W. Wattenberg ,et al. published in Cancer research 1979 . The protocol is quite similar as you suggested in De Coaster et al. I would like to tell that in last 2-3 weeks some tumors were palpated but it took around 32 weeks after DMBA treatment. In next experiment, I'll be thankful, if you suggest me any good article and a good procedure as you experienced , through which I can induce tumors in less duration.
Thanks again for your interest and anticipations of your suggestions!
Hello Shankar, I have experience with DMBA and female Sprague Dawley rats. I administer DMBA at 55 days of age, 15 mg per rat (0,5 ml) dissolved in a few drops of benzyl alcohol and sunflower oil. One month later the first tumors appear. These tumors are hormone dependent, so if you have ovariectomized rats it is probably that you have low or none incidences. I hope you have luck with that. Best regards.
hello Corina,
Thanks for your suggestion
Can you pls tell me how many rats you find out the appearance of tumors. if you can tell this It could be helpful to me take number of rats accordingly.
Hello Shankar, I made a group of 5 rats with intact ovaries and all of them (100%) developed tumors between 53-170 days after DMBA administration. Now I am running another experiment to increase the number of rats of the group and on day 55 the first tumor appeared. Good luck and I am at your disposal for anything you need.
In this model, did you observe mortality after DMBA administration ? If yes, what was the is the percentage and when did it happen ?
Yes, I got 20% of total rats are died after 2-6 weeks of DMBA treatment.
I come back to the rat model of mammary tumors.Have you seen localized tumors outside of the mammary glands ? In our animal model, tumors seem to grow above the forelegs. Did you observe this phenomenom ?
Dear Benoit, I have also observed the same phenomena in some rats and in another rats, it was also localized nearby mammary glands at initial stage. After some time, I also got swelling in gland area.
Dear Shankar, did you try to infect the rats by the mouse mammary tumor virus (MMTV)? You can hope to get success, if you will inject splenocytes of C3H mice (with high incidence of mammary tumors) into newborn rats.
if you can use a mouse model in place of rat model you can develope the mammary tumor by injecting T47D or you can also try with other cell lines such as MCF-7 and MDAMB-231,cell line at mammary fat pad of the mice.
You can inject the mammary fat pad with rat mammary tumor cell lines tumor will develop within a week.
- Badges
- Science topic
- Similar topics
- Medicine
- Disease
- Disease Models
- Animal Models
- Rodent Models
- Mouse Models