How to separate dead cells in a suspension culture of protozoans particularly Tryapnosomes?

I am working on Tryapanosomes epimastigotes. It grows in the suspension media and I want to select some tranfectant cells after selecting with antibiotics. I have to centrifuge for at least...

04 May 2018 8,936 0 View

How to calculate combined AUC of multiple markers using SPSS or any freely available softwares ?

I wanted to calculate the combined AUC as well as sensitivity and specificity of multiple markers of specific disease. Does anybody help me to work on this. Thanks

03 April 2018 5,474 4 View

I am looking to inhibit Lukemia inhibitory factor receptor (LIFR) for some of my study and I am searching for chemical inhibitors, any suggestions?

01 February 2017 9,498 0 View

Does N-acetyl cysteine play biased role in cancer ?

08 September 2014 3,665 4 View

How to convert GI number to uniprot accession number of protein ?

I desire to convert GI number to uniprot /GO accession number of protein through available online free tools for mapping network pathways as well as cellular localisation.

08 September 2013 1,564 2 View

Does the pooling of unequal number of biological samples but equal loading of differential proteomics may affect the reliability of results?

usually some category in clinical samples is very tedious to equalize with control or healthy because it could be time taking. In most of articles delivers the small sample size equalize with...

06 July 2013 1,228 4 View

What is the best method of nonsurgical blood collection from rats?

I desire to collect blood from rats. I'm able get blood but it is very small in quantity. Also RBCs are lysed after centrifuging at 1200g for 20min. Can anybody suggest the best method for...

03 April 2013 442 5 View

Does anybody have experience with older commassie stained gel's spots identification through mass spectroscopy?

I wish to know how older spots of commassie stained gel can be identified. I have older gels more than six months. I was not able to identify through MALDI-TOF. Meanwhile I was able to know the...

03 April 2013 5,575 4 View

How can I develop a rat model for mammary tumours in normal rats without inducing the tumour chemically?

I followed a method for the development of mammary tumours in spargue dawley rats by using DMBA. According to the article, after 15-20 weeks for the tumour growth could have shown. It is more than...

01 February 2013 7,617 15 View

How to isolate proteins from PVDF blotting paper for MALDI-TOF analysis?

Does anyone have experience with identification of protein from blotting membrane? I tried to follow several methods, but I have found very less robustness and high protein loss during the...

10 November 2012 4,926 9 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View