I desire to collect blood from rats. I'm able get blood but it is very small in quantity. Also RBCs are lysed after centrifuging at 1200g for 20min. Can anybody suggest the best method for collection of blood from a rat vein with a needle?
Depending on the quantity of blood needed the tail vein is a good choice. Cardiocentesis, jugular, and saphenous are better if you need larger quantities.
Collect it from the retro orbital plexus. You can anesthetize the rat with anesthetic ether and then collect the blood using a fine capillary. By far this is the best method I have used because tail vein puncture gives blood but in a lesser quantity and is quite tedious also. Yes it is good if you need only 1 or 2 drops for testing blood glucose levels using Accu-check.
Thanks so much for your suggestions,
Right, it very tedious but I've collected 1ml of blood from tail vein in EDTA vial from rats of weight 300g. But the problem is I could not able to collect good sera after centrifugation. can you suggest some more to resolve this?
Try collecting it from the retro orbital plexus. Serum is easily separated at 2000 g for 20 min.
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