I am searching some inhibitor of LIFR to study some molecular interactions, I desire to see whether any chemicals have potential to inhibit LIFR. I would be also thankful any suggestions.
I am working on Tryapanosomes epimastigotes. It grows in the suspension media and I want to select some tranfectant cells after selecting with antibiotics. I have to centrifuge for at least...
04 May 2018 9,088 0 View
I wanted to calculate the combined AUC as well as sensitivity and specificity of multiple markers of specific disease. Does anybody help me to work on this. Thanks
03 April 2018 5,745 4 View
I desired to learn out from the oncologist working particularly on NAC like antioxidant and cancer. My query is related to NAC, since Jan 2014, Nature news published an article entitled...
08 September 2014 3,806 4 View
I desire to convert GI number to uniprot /GO accession number of protein through available online free tools for mapping network pathways as well as cellular localisation.
08 September 2013 1,794 2 View
usually some category in clinical samples is very tedious to equalize with control or healthy because it could be time taking. In most of articles delivers the small sample size equalize with...
06 July 2013 1,387 4 View
I've basic queries that many demo version softwares are available online for few days validity. can the analysed data from that version of software are acceptable for any journal by acknowledgement.
03 April 2013 6,593 2 View
I desire to collect blood from rats. I'm able get blood but it is very small in quantity. Also RBCs are lysed after centrifuging at 1200g for 20min. Can anybody suggest the best method for...
03 April 2013 572 5 View
I wish to know how older spots of commassie stained gel can be identified. I have older gels more than six months. I was not able to identify through MALDI-TOF. Meanwhile I was able to know the...
03 April 2013 5,689 4 View
I followed a method for the development of mammary tumours in spargue dawley rats by using DMBA. According to the article, after 15-20 weeks for the tumour growth could have shown. It is more than...
01 February 2013 7,773 15 View
Does anyone have experience with identification of protein from blotting membrane? I tried to follow several methods, but I have found very less robustness and high protein loss during the...
10 November 2012 5,074 9 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View