How to separate dead cells in a suspension culture of protozoans particularly Tryapnosomes?

I am working on Tryapanosomes epimastigotes. It grows in the suspension media and I want to select some tranfectant cells after selecting with antibiotics. I have to centrifuge for at least...

04 May 2018 8,936 0 View

How to calculate combined AUC of multiple markers using SPSS or any freely available softwares ?

I wanted to calculate the combined AUC as well as sensitivity and specificity of multiple markers of specific disease. Does anybody help me to work on this. Thanks

03 April 2018 5,474 4 View

Does N-acetyl cysteine play biased role in cancer ?

08 September 2014 3,665 4 View

How to convert GI number to uniprot accession number of protein ?

I desire to convert GI number to uniprot /GO accession number of protein through available online free tools for mapping network pathways as well as cellular localisation.

08 September 2013 1,564 2 View

Does the pooling of unequal number of biological samples but equal loading of differential proteomics may affect the reliability of results?

usually some category in clinical samples is very tedious to equalize with control or healthy because it could be time taking. In most of articles delivers the small sample size equalize with...

06 July 2013 1,228 4 View

Can the demo version software would be acceptable for publishing of the analysed raw data?

I've basic queries that many demo version softwares are available online for few days validity. can the analysed data from that version of software are acceptable for any journal by acknowledgement.

03 April 2013 6,468 2 View

What is the best method of nonsurgical blood collection from rats?

I desire to collect blood from rats. I'm able get blood but it is very small in quantity. Also RBCs are lysed after centrifuging at 1200g for 20min. Can anybody suggest the best method for...

03 April 2013 442 5 View

Does anybody have experience with older commassie stained gel's spots identification through mass spectroscopy?

I wish to know how older spots of commassie stained gel can be identified. I have older gels more than six months. I was not able to identify through MALDI-TOF. Meanwhile I was able to know the...

03 April 2013 5,575 4 View

How can I develop a rat model for mammary tumours in normal rats without inducing the tumour chemically?

I followed a method for the development of mammary tumours in spargue dawley rats by using DMBA. According to the article, after 15-20 weeks for the tumour growth could have shown. It is more than...

01 February 2013 7,617 15 View

How to isolate proteins from PVDF blotting paper for MALDI-TOF analysis?

Does anyone have experience with identification of protein from blotting membrane? I tried to follow several methods, but I have found very less robustness and high protein loss during the...

10 November 2012 4,926 9 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View