How to separate dead cells in a suspension culture of protozoans particularly Tryapnosomes?

I am working on Tryapanosomes epimastigotes. It grows in the suspension media and I want to select some tranfectant cells after selecting with antibiotics. I have to centrifuge for at least...

04 May 2018 8,936 0 View

I am looking to inhibit Lukemia inhibitory factor receptor (LIFR) for some of my study and I am searching for chemical inhibitors, any suggestions?

01 February 2017 9,498 0 View

Does N-acetyl cysteine play biased role in cancer ?

08 September 2014 3,665 4 View

How to convert GI number to uniprot accession number of protein ?

I desire to convert GI number to uniprot /GO accession number of protein through available online free tools for mapping network pathways as well as cellular localisation.

08 September 2013 1,564 2 View

Does the pooling of unequal number of biological samples but equal loading of differential proteomics may affect the reliability of results?

usually some category in clinical samples is very tedious to equalize with control or healthy because it could be time taking. In most of articles delivers the small sample size equalize with...

06 July 2013 1,228 4 View

Can the demo version software would be acceptable for publishing of the analysed raw data?

I've basic queries that many demo version softwares are available online for few days validity. can the analysed data from that version of software are acceptable for any journal by acknowledgement.

03 April 2013 6,468 2 View

What is the best method of nonsurgical blood collection from rats?

I desire to collect blood from rats. I'm able get blood but it is very small in quantity. Also RBCs are lysed after centrifuging at 1200g for 20min. Can anybody suggest the best method for...

03 April 2013 442 5 View

Does anybody have experience with older commassie stained gel's spots identification through mass spectroscopy?

I wish to know how older spots of commassie stained gel can be identified. I have older gels more than six months. I was not able to identify through MALDI-TOF. Meanwhile I was able to know the...

03 April 2013 5,575 4 View

How can I develop a rat model for mammary tumours in normal rats without inducing the tumour chemically?

I followed a method for the development of mammary tumours in spargue dawley rats by using DMBA. According to the article, after 15-20 weeks for the tumour growth could have shown. It is more than...

01 February 2013 7,617 15 View

How to isolate proteins from PVDF blotting paper for MALDI-TOF analysis?

Does anyone have experience with identification of protein from blotting membrane? I tried to follow several methods, but I have found very less robustness and high protein loss during the...

10 November 2012 4,926 9 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View