Shankar Suman

11 Questions 24 Answers 0 Followers

Questions related from Shankar Suman

How to separate dead cells in a suspension culture of protozoans particularly Tryapnosomes?

I am working on Tryapanosomes epimastigotes. It grows in the suspension media and I want to select some tranfectant cells after selecting with antibiotics. I have to centrifuge for at least...

05 May 2018 9,044 0 View

How to calculate combined AUC of multiple markers using SPSS or any freely available softwares ?

I wanted to calculate the combined AUC as well as sensitivity and specificity of multiple markers of specific disease. Does anybody help me to work on this. Thanks

04 April 2018 5,707 4 View

I am looking to inhibit Lukemia inhibitory factor receptor (LIFR) for some of my study and I am searching for chemical inhibitors, any suggestions?

I am searching some inhibitor of LIFR to study some molecular interactions, I desire to see whether any chemicals have potential to inhibit LIFR. I would be also thankful any suggestions.

02 February 2017 9,579 0 View

Does N-acetyl cysteine play biased role in cancer ?

I desired to learn out from the oncologist working particularly on NAC like antioxidant and cancer. My query is related to NAC, since Jan 2014, Nature news published an article entitled...

09 September 2014 3,760 4 View

How to convert GI number to uniprot accession number of protein ?

I desire to convert GI number to uniprot /GO accession number of protein through available online free tools for mapping network pathways as well as cellular localisation.

09 September 2013 1,719 2 View

Does the pooling of unequal number of biological samples but equal loading of differential proteomics may affect the reliability of results?

usually some category in clinical samples is very tedious to equalize with control or healthy because it could be time taking. In most of articles delivers the small sample size equalize with...

07 July 2013 1,333 4 View

Can the demo version software would be acceptable for publishing of the analysed raw data?

I've basic queries that many demo version softwares are available online for few days validity. can the analysed data from that version of software are acceptable for any journal by acknowledgement.

04 April 2013 6,551 2 View

What is the best method of nonsurgical blood collection from rats?

I desire to collect blood from rats. I'm able get blood but it is very small in quantity. Also RBCs are lysed after centrifuging at 1200g for 20min. Can anybody suggest the best method for...

04 April 2013 521 5 View

Does anybody have experience with older commassie stained gel's spots identification through mass spectroscopy?

I wish to know how older spots of commassie stained gel can be identified. I have older gels more than six months. I was not able to identify through MALDI-TOF. Meanwhile I was able to know the...

04 April 2013 5,648 4 View

How can I develop a rat model for mammary tumours in normal rats without inducing the tumour chemically?

I followed a method for the development of mammary tumours in spargue dawley rats by using DMBA. According to the article, after 15-20 weeks for the tumour growth could have shown. It is more than...

02 February 2013 7,738 15 View

How to isolate proteins from PVDF blotting paper for MALDI-TOF analysis?

Does anyone have experience with identification of protein from blotting membrane? I tried to follow several methods, but I have found very less robustness and high protein loss during the...

11 November 2012 5,026 9 View