usually some category in clinical samples is very tedious to equalize with control or healthy because it could be time taking. In most of articles delivers the small sample size equalize with different conditions, But large samples size have differed in their number of samples in pooling specially for clinical samples. Does there is a great significance of taking number of samples equally before for proteomics experiment.?
different biological samples have different protein concentration, which bioloogical sample you are interested in. serum usually have a protein concentration of 60-80g/L, with 12 proteins constituting 95% of the protein concentration, these same aspects are also found in other biological fluids except urine. although perforing protein concentration is tedious, using the tubes, nowadays all the protein detection kits are available for 96 well format to do the protein concentrations.
for a reliable data equal loading should be done prior to any mudpit proteomics. this would remove any doubts on presence of any peak in a few (high conc) samples vs samples with low protein concentration.
Thanks for answering my query. you r exactly telling the problem. I've interested to work on serum sample with depletion. I would like to know that whether in two or more cohorts of same biological samples in unequal number should be compared by pooling equally quantitative dividers and equal loading.
if you are performing chicken antibody based depletion, there is a huge variability from run to run. I would suggest proteominer kit from biorad, which is somewhat better way to go. here is a paper talking about various factors in proteomics study from our lab.http://www.ncbi.nlm.nih.gov/pubmed/20663466
If i understand correctly you are talking about matched samples in terms of loading and concentrations. yes the samples have to be matched for protein concentration and demographics
- Badges
- Science topic
- Similar topics
- Chemistry
- General Biochemistry
- Proteomics