I wish to know how older spots of commassie stained gel can be identified. I have older gels more than six months. I was not able to identify through MALDI-TOF. Meanwhile I was able to know the spots of the fresh gel through this. I would like to add that I stored all gels in 1% glycerol. Can anybody suggest the best protocol which can be helpful for me? And also what solution I use for gels storage in future identification purpose?
Dear Dr. Albertin,
Thanks so much for reply. Have you experienced about the storage of picked spots at -20°C for long term and how much variability u would observed after that. if there are not much alteration, it be a much convenient for me.
Thanks Dr. Albertin,
I'll try to follow your suggestions. I'll be again contact in future after testing this. Thanks so much.
Dear Shankar Suman
I stored my gels in plastic covers with deionized water for long time in 4°C, in dark. Also, there is a machine that pack and dry the 2DE gels for very long time storing.
Dear Sepehrimanesh,
Thanks for your reply.
which plastic cover you use for storage. Can you explain a bit more of your protocol?
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