How can I add sodium chloride in 1X PBS to 450mM?

09 September 2017 0 9K Report

Oh, greetings.

I plan to experiment for 'stringency of DNA'. so I add some sodium chlodire to PBS. and make 1X PBS(450mM NaCl). It same to concentration of NaCl in 3X SSC.

But in 1X PBS, I buy made buffer, so added 137mM NaCl. then How can I add sodium chloride for make 450mM?

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