What is 5X binding buffer?

Kyungsun Ha @Kyungsun_Ha

01 January 2019 0 5K Report

I saw 5X bingind buffer maded by;

100mM HEPES, pH 7.6
5mM EDTA
50mM ammonium sulfate
5mM DTT
1% Tween-20
150mM potassium chloride

which reaction helps this buffer? I don't consider to this buffer participates in which experiment by name.

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Science method

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