Greetings. Here is sunday...

In tris-glycine buffer, there are either include 1% SDS or not. I used former but many seller selling both Tris-glycine with SDS and without SDS. SDS is set charge of protein by negative, I know.

Without SDS, what sets charge of protein for negative? sellers said just 'for SDS-PAGE' both w/ SDS and w/o SDS, I don't know what's differ.