24 Questions 13 Answers 0 Followers
Questions related from Kyungsun Ha
Greetings. I cannot find about each component's role of CHURCH buffer at southern blotting(detail, pre-hybridization). I did DNA microarray but never have ever southern blot. In microarray,...
01 January 2019 968 0 View
Greetings. I extracted RNA at mouse liver and human blood, but degradation occurs. I think, at mouse liver, too hard to measuring 50mg liver(I am newbie for animal tissue preparation) so spend...
01 January 2019 4,712 0 View
Greetings. I heard about when make buffer include Tris, set pH using concentrated hydrochloric acid(37%). but SDS-PAGE, loading buffer(10X), Tris-glycine, what I heard set pH for 8.3, use only...
01 January 2019 4,019 3 View
Greetings. Here is sunday... In tris-glycine buffer, there are either include 1% SDS or not. I used former but many seller selling both Tris-glycine with SDS and without SDS. SDS is set charge...
01 January 2019 1,267 0 View
Greetings. Before question, I never did southern/nothern blot. (eastern too) During search buffer, I found Alkaline transfer buffer and knew it uses southern blot(alkaline method). This buffer...
01 January 2019 9,012 0 View
I saw 5X bingind buffer maded by; 100mM HEPES, pH 7.6 5mM EDTA 50mM ammonium sulfate 5mM DTT 1% Tween-20 150mM potassium chloride which reaction helps this buffer? I don't consider to this...
01 January 2019 4,980 0 View
Greetings. In image... left:ladder(start with 750bp) middle: PCR product with exonuclease III treated right(3rd lane): PCR product without exonuclease III treated there's a problem. PCR...
11 November 2017 9,902 12 View
I read a paper about PEG gel, . in this paper, supplymentary fig 10 is storage stability test. This particle maded by polyethylene glycol and store in 4 degree, but storage test is run at -20...
10 October 2017 2,875 0 View
Oh, greetings. I plan to experiment for 'stringency of DNA'. so I add some sodium chlodire to PBS. and make 1X PBS(450mM NaCl). It same to concentration of NaCl in 3X SSC. But in 1X PBS, I buy...
09 September 2017 8,754 0 View
Greetings. Experiments using microparticles are in progress. There are particles with multiple compositions and features, advisors want to compare the features of this particle finely, but I can...
09 September 2017 577 0 View
Guten morgen.(means 'Good mornong' in Deutsch) Recently I read a paper entitled "Circulating Tumor DNA Mutation Profiling by Targeted Next Generation Sequencing Provides Guidance for Personalized...
09 September 2017 5,111 1 View
In NHS/EDC protocol, there are two buffers. coupling buffer and activation buffer. (+washing buffer) And, I will conjugate PEG-COOH to amine-modified oligonucleotide. In the NHS / EDC reaction...
08 August 2017 1,409 2 View
Greetings. Actually, I will start new experiment. but before exp, advisor said "you have to study how NH2 modified DNA coupling(=conjugate). " and, DNA is negative charge(by its PO4- on backbone),...
07 July 2017 8,439 3 View
I want to find about molecular diagnostics, especially technique's evolution(history), and techniques that using nowadays.. but I cannot find something... Can you resommend article or websites...
06 June 2017 7,403 0 View
Greetings.May I ask about something for my experiment? At first picture, three band(of each channel) have vivid blask. but surong experiment, channel 2(at middle) band disappeared by hal, and...
06 June 2017 4,278 1 View
Greetings! I run microarray at many condition, so I want to know many things. 1. Why we have to hybridization at 42 degree? I ran experiment, hybridization durong O/N(16~20h), it was good...
04 April 2017 1,598 1 View
I solute both target(which adhered with glass) and probe in 50% DMSO. But, when I spotting DNA and baking in 80 degree, it spreaded and mixed. DMSO has low surface tension? where you solute your...
03 March 2017 695 0 View
As you see photo below, 3rd and 6th dish are distorted by heat(3rd : w/o water, 6th : w/ water). Oh my... other dish isn't distorted seriously, I think because it not touched by oven's...
03 March 2017 6,534 6 View
Greetings! It's Monday.. Actually, I ruined my first microarray because 'poor signal'... I find where I printed DNA very very difficultly, but it wasn't shining. label is cy3, concentration is...
03 March 2017 4,456 2 View
Greetings! I want to know about glass petri dish.. I used plastic petri dish at baking step of microarray, but it was distorted... so I want to use glass dish. But, is it Okay I reuse that dishes?...
03 March 2017 8,392 1 View
all of hybridization buffers are preheated(pre-hybridization, hybridization, washing). but why we have to preheat it?
03 March 2017 441 4 View
Greetings! Today I come to ask something.. I will run experiment PI staning for microarrayed slide, because I cannot see fluorescence(cy3). but there are protocol only for animal cell... my DNA...
03 March 2017 7,574 2 View
Greetings! ㅇㅅㅇ/ Suddnly, I have a curious question. It's very weaken, but when I baked at 80 degree at dry oven shows fluorescence(cy3 is green but they changed for easily view fluorescence), but,...
03 March 2017 9,563 0 View
I made pre-hybridization buffer. it contains 5X SSC/0.1% SDS/1% BSA, I maded 500ml solution. but 5g of albumin cannot solute entirely in DIW, I saw some BSA that cannot entirely solved. How can I...
03 March 2017 8,413 5 View
