Is there an influence of terminal cardiac puncture on subsequent brain analysis?

We want to analyse protein expression and metabolomics in mouse brains. The sampling method is snap-freezing in liquid nitrogen. Secondary outcome is blood analysis, which we plan to sample by...

08 December 2020 5,028 2 View

QRT-PCR: DNAse treatment and/or exon overlapping primer required?

How concerned do I have to be about a qRT-PCR experiment in that RNA was not treated with DNAse prior RT and primer pairs are within one exon? Is this a no-no-no or is this usually fine since cDNA...

09 March 2020 7,367 3 View

Ask for help about wannier90, hopping matrix elements are not complex conjugate?

Dear All, I calculate band structure and hopping matrix of monolayer Graphene by using wannier90-2.0.1. There are two atoms in one unit cell, the following is input information : INCAR :...

26 February 2020 4,885 1 View

Why is there a change in EFL measurement in liquid medium?

Hello Everyone, This is regarding the EFL change of a biconvex lens. Can someone explain to me the reason for a change in the measurement of a boconvex lens in water when measured with a time gap...

07 August 2019 7,610 1 View

Has anyone explored the links between yoga and flourishing?

I'm a yoga teacher and MAPP student looking to write my dissertation on the links between yoga practice and flourishing/wellbeing. I'm in the very early stages of looking at this topic.

22 November 2018 3,731 2 View

Anyone has a good recommendation for a study examining the psychometrics properties of CDI-2?

I can only find psychometrics examination of the CDI (the previous edition). I would greatly appreciate it if anyone can share a good study looking at the psychometrics properties of the child's...

08 April 2018 1,667 3 View

How have others addressed a large number of 'Not applicable' answers in survey responses?

I have a number of variables being used in the development of a scale. In my survey analysis I've deemed the 'Not applicable' (NA) selections as missing so they are not used in the analysis -...

18 December 2014 5,945 11 View

What are the reasons a PCR that once functioned, is not working anymore?

Last week two Test-PCRs for a new set of primers worked quite well and i got one defined product band. One week later i am not able to get any product again. I exchanged all materials, also the...

25 November 2014 5,733 72 View

Can someone advise on PCR contamination?

I am trying to do some basic mouse genotyping, but am having problem with a thin band being seen in the water. I have 4 other genes that I routinely genotype and never have had this issue. I use...

29 May 2014 4,114 5 View

Necessity of "Neighbourhood Policing"

Is there a real necessity and emphasis for a "neighbourhood policing" approach to be adopted nationally across all forces? How big of an impact does it have on crime? Public perception...

01 January 1970 492 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View