We want to analyse protein expression and metabolomics in mouse brains. The sampling method is snap-freezing in liquid nitrogen. Secondary outcome is blood analysis, which we plan to sample by cardiac puncture under terminal anesthesia.
Now we wonder whether the method of death can have any effects on the brain tissue data. Of course, blood withdrawal will cause hypoxia, which in turn will cause transcriptional changes etc.
But at what time frame is this to be expected?
Any advice is appreciated!
I would imagine that the cardiac puncture will have no additive effect beyond the effect that terminal anaesthesia would already have. As long as you don't linger with the cardia puncture.
Hello Simon N Waddington ,
thank you for your answer, this is reassuring.
Could your perhaps elaborate on "don't linger"? We decided to set us a time limit for blood withdrawal, but are unsure of what would be suitable. 5 minutes?
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