Has anyone induced EGFR mutant cells with macrophages?

12 December 2014 0 6K Report

If you have induced either PC9 or HCC827 cells with macrophages, then what kind of morphological changes have you seen in the cell?

Does this change in morphology affect migration, wound healing and invasion potential of the cell?

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Is sonication a preferable method in preparing protein lysate for immuno blot?

What kind of differences can be seen in the sample with or without sonication?

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Does monocyte chemoattractant protein-1 (MCP-1) have the effect of attracting cancer cells (PC9) or not?

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