Could anyone suggest me protocol for ammonium sulfate protein precipitation for SDS-gel running?

I am trying to check collagen in growth media of fibroblasts.

09 October 2018 1,919 0 View

What exactly is protocol for SirCol Collagen Assay?

Sircol Collagen Assay on Mouse Embryonic Fibroblast (MEFs).

03 April 2018 2,424 1 View

Does anyone have worked on immunoblotting of Sec61-Gamma subunit protein (7 kDa)?

I wanted to check translocon proteins (Sec61 alpha, beta, gamma) in endoplasmic reticulm. I got band for alpha subunit protein. However I could not band for beta sub-unit (15 kDa) and gamma...

08 September 2016 9,417 2 View

What are the points to be considered in immunoblot to detect protein size of 7.5 kDa?

06 July 2016 7,742 3 View

How can I make immunoblot bands straight and not curved?

Immunoblot bands of protein size below 20KDa (19, 15, 12) becomes curved, I use PC9 cell. However bands for larger protein size has no problem. I use 12% and 15% SDS Gel and gel running at 100...

03 April 2015 5,043 1 View

What is the best method to explain number of invaded cells through matrigel coated membrane in invasion assay?

I performed invasion assay for PC9 cell and I found many way of expressing invaded cells through the matrigel in published papers: 1) simply the number of invaded cells 2) % of invaded cells and...

02 March 2015 3,031 2 View

Is sonication a preferable method in preparing protein lysate for immuno blot?

02 March 2015 9,756 4 View

Has anyone induced EGFR mutant cells with macrophages?

11 December 2014 5,615 0 View

Does monocyte chemoattractant protein-1 (MCP-1) have the effect of attracting cancer cells (PC9) or not?

10 November 2014 3,751 4 View

Can anybody help me with the migration assay protocol for PC9 cell?

If there is any specific chemoattracant for the PC9 cell?

10 November 2014 3,739 5 View

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02 March 2021 3,060 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View