Subodh Sharma

12 Questions 7 Answers 0 Followers

Questions related from Subodh Sharma

Could anyone suggest me protocol for ammonium sulfate protein precipitation for SDS-gel running?

I am trying to check collagen in growth media of fibroblasts.

10 October 2018 1,971 0 View

What exactly is protocol for SirCol Collagen Assay?

Sircol Collagen Assay on Mouse Embryonic Fibroblast (MEFs).

04 April 2018 2,494 1 View

Does anyone have worked on immunoblotting of Sec61-Gamma subunit protein (7 kDa)?

I wanted to check translocon proteins (Sec61 alpha, beta, gamma) in endoplasmic reticulm. I got band for alpha subunit protein. However I could not band for beta sub-unit (15 kDa) and gamma...

09 September 2016 9,501 2 View

What are the points to be considered in immunoblot to detect protein size of 7.5 kDa?

I am trying to detect Sec61-gamma with protein size 7.5 kDa. Does anybody have suggestions for me on working with such small protein size? What advice you give me regarding the process?

07 July 2016 7,811 3 View

How can I make immunoblot bands straight and not curved?

Immunoblot bands of protein size below 20KDa (19, 15, 12) becomes curved, I use PC9 cell. However bands for larger protein size has no problem. I use 12% and 15% SDS Gel and gel running at 100...

04 April 2015 5,118 1 View

Can anyone make me understand the mechanism of DNA fregmentation done for the cell apoptosis?

I am wondering..what might me the mechanism behind DNA fregmentation during cell apoptosis. If the dna seems condensed and the staining is more brighter than the dna in control cell, what does it...

04 April 2015 353 0 View

Is sonication a preferable method in preparing protein lysate for immuno blot?

What kind of differences can be seen in the sample with or without sonication?

03 March 2015 9,833 4 View

What is the best method to explain number of invaded cells through matrigel coated membrane in invasion assay?

I performed invasion assay for PC9 cell and I found many way of expressing invaded cells through the matrigel in published papers: 1) simply the number of invaded cells 2) % of invaded cells and...

03 March 2015 3,119 2 View

Has anyone induced EGFR mutant cells with macrophages?

If you have induced either PC9 or HCC827 cells with macrophages, then what kind of morphological changes have you seen in the cell? Does this change in morphology affect migration, wound healing...

12 December 2014 5,681 0 View

Which fixative is best for PC9 cells: 4% formalin or 100% methanol?

I read in papers; some have used 4% formalin and some have used 100% methanol. Further, in case of PC9 cell staining with DAPI or Hoecast 3332, how important is permeabilization step? Is the step...

11 November 2014 5,822 3 View

Can anybody help me with the migration assay protocol for PC9 cell?

If there is any specific chemoattracant for the PC9 cell?

11 November 2014 3,797 5 View

Does monocyte chemoattractant protein-1 (MCP-1) have the effect of attracting cancer cells (PC9) or not?

PC9 is EGFR mutant non small cell lung cancer cell (adenocarcinoma)

11 November 2014 3,813 4 View