I am trying to check collagen in growth media of fibroblasts.
Sircol Collagen Assay on Mouse Embryonic Fibroblast (MEFs).
03 April 2018 2,541 1 View
I wanted to check translocon proteins (Sec61 alpha, beta, gamma) in endoplasmic reticulm. I got band for alpha subunit protein. However I could not band for beta sub-unit (15 kDa) and gamma...
08 September 2016 9,558 2 View
I am trying to detect Sec61-gamma with protein size 7.5 kDa. Does anybody have suggestions for me on working with such small protein size? What advice you give me regarding the process?
06 July 2016 7,865 3 View
I am wondering..what might me the mechanism behind DNA fregmentation during cell apoptosis. If the dna seems condensed and the staining is more brighter than the dna in control cell, what does it...
03 April 2015 412 0 View
Immunoblot bands of protein size below 20KDa (19, 15, 12) becomes curved, I use PC9 cell. However bands for larger protein size has no problem. I use 12% and 15% SDS Gel and gel running at 100...
03 April 2015 5,166 1 View
I performed invasion assay for PC9 cell and I found many way of expressing invaded cells through the matrigel in published papers: 1) simply the number of invaded cells 2) % of invaded cells and...
02 March 2015 3,173 2 View
What kind of differences can be seen in the sample with or without sonication?
02 March 2015 9,890 4 View
If you have induced either PC9 or HCC827 cells with macrophages, then what kind of morphological changes have you seen in the cell? Does this change in morphology affect migration, wound healing...
11 December 2014 5,724 0 View
PC9 is EGFR mutant non small cell lung cancer cell (adenocarcinoma)
10 November 2014 3,863 4 View
If there is any specific chemoattracant for the PC9 cell?
10 November 2014 3,859 5 View
Dear QE-users, In the method where full MS positive mode and PRM mode are used, we always get an incorrect auxiliary gas reading (41 instead of 25). This only happens in this method; other...
06 August 2024 4,953 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hello guys! Do you have experience running a Oaxaca-Blinder decomposition on R applying person weights. How do you suggest doing it? I have a variable PERWT which gives more information on how...
04 August 2024 6,033 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello Everyone I have a question about structure for connectivity analysis on sources. My goal: preprocess and cut data into trials create headmodels, using template MRI file perform source...
30 July 2024 2,744 1 View
When I run autogrid4 it says: autogrid4: ERROR: Unknown receptor type: "Cr" -- Add parameters for it to the parameter library first! Look forward to your reply.Thank you so much!
29 July 2024 488 0 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
I am trying to run a restart analysis, which imports deformed configurations of parts from a generated ODB file. It runs fine in Windows OS but when I try to run it in Linux OS, it is giving some...
29 July 2024 9,572 3 View
Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...
28 July 2024 6,553 1 View
