What exactly is protocol for SirCol Collagen Assay?

Sircol Collagen Assay on Mouse Embryonic Fibroblast (MEFs).

03 April 2018 2,424 1 View

Does anyone have worked on immunoblotting of Sec61-Gamma subunit protein (7 kDa)?

I wanted to check translocon proteins (Sec61 alpha, beta, gamma) in endoplasmic reticulm. I got band for alpha subunit protein. However I could not band for beta sub-unit (15 kDa) and gamma...

08 September 2016 9,417 2 View

What are the points to be considered in immunoblot to detect protein size of 7.5 kDa?

06 July 2016 7,742 3 View

Can anyone make me understand the mechanism of DNA fregmentation done for the cell apoptosis?

I am wondering..what might me the mechanism behind DNA fregmentation during cell apoptosis. If the dna seems condensed and the staining is more brighter than the dna in control cell, what does it...

03 April 2015 288 0 View

How can I make immunoblot bands straight and not curved?

Immunoblot bands of protein size below 20KDa (19, 15, 12) becomes curved, I use PC9 cell. However bands for larger protein size has no problem. I use 12% and 15% SDS Gel and gel running at 100...

03 April 2015 5,043 1 View

What is the best method to explain number of invaded cells through matrigel coated membrane in invasion assay?

I performed invasion assay for PC9 cell and I found many way of expressing invaded cells through the matrigel in published papers: 1) simply the number of invaded cells 2) % of invaded cells and...

02 March 2015 3,031 2 View

Is sonication a preferable method in preparing protein lysate for immuno blot?

02 March 2015 9,756 4 View

Has anyone induced EGFR mutant cells with macrophages?

11 December 2014 5,615 0 View

Does monocyte chemoattractant protein-1 (MCP-1) have the effect of attracting cancer cells (PC9) or not?

10 November 2014 3,751 4 View

Can anybody help me with the migration assay protocol for PC9 cell?

If there is any specific chemoattracant for the PC9 cell?

10 November 2014 3,739 5 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View