For how long should a mammalian cell line be kept in -80 degree C after transferring it from liq nitrogen to culture the cell in media?

03 July 2020 1 10K Report

for how long is it ideal to keep in -80 degree celsius after transferring from liq nitrogen to culture the cell

I agree with Oliver. Ideally, you should immediately remove from liquid Nitrogen, place on water bath, follow procedures to remove the "freezing media" and plate the cells.

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