for how long is it ideal to keep in -80 degree celsius after transferring from liq nitrogen to culture the cell
I agree with Oliver. Ideally, you should immediately remove from liquid Nitrogen, place on water bath, follow procedures to remove the "freezing media" and plate the cells.
We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...
02 March 2021 3,713 2 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
28 February 2021 7,738 3 View
I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
28 February 2021 274 2 View
In Terrset, an error encountered in executing Spatial Decision Modeller (MCE). During execution or run the MCE a massage come on the screen that reflects "Your columns and rows are not same" , I...
07 December 2020 1,542 1 View
Can you please provide me the reference
17 November 2020 6,128 2 View
I would like to see RNA-protein interaction following transcription process inside the cell. I am wondering if there is any protocol similar to protein co-immunoprecipitation,which can bind the...
27 October 2020 2,940 3 View
I have already been read many articles and found some methods but those are not cleared to me. Can you please suggest me how to generate different LST map of each land classes using ArcGIS? Or...
25 October 2020 5,978 5 View
I would like to assess how the reasons of migration varies across type of environmental migrants, and therefore, how it could be useful for modelling adaptation strategies for the future migrants.
04 October 2020 5,435 7 View
1. LATEST RESEARCH TREND ON CRYOGENIC MACHINING 2. POLYMER BEHAVIOR BELOW GLASS TRANSITION TEMPERATURE
23 January 2020 4,482 2 View
I do research in Kolkata, India?
06 October 2019 9,421 3 View
02 March 2021 3,060 3 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
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Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...
01 March 2021 9,911 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
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Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?
01 March 2021 9,785 3 View
I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...
01 March 2021 6,450 5 View