Argha Mario Mallick

19 Questions 5 Answers 0 Followers

Questions related from Argha Mario Mallick

Can I culture suspension cell line (Jurkat) in flasks used for adherent cell line?

In our lab we have cell culture wares for adherent cell lines which are coated. Can I use the same flask for culturing suspension cell line (Jurkat)?

26 June 2023 4,360 2 View

What are few normal cell lines to test toxicity on normal cells of designed anti cancer drug?

we have synthesized anticancer drugs which are toxic to cancer cells. but it is desirable that such drugs are non toxic to normal cells. pls provide me a list of normal cell lines where such...

14 March 2023 2,475 4 View

Can binding constant of two proteins be determined by ELISA?

What is minimum amount of the 2 proteins we need for getting a correct result ?

21 February 2022 2,708 3 View

Any good paper for the explanation of basis of combination therapy for cancer, especially TNBC?

There are lots of reviews on combination of drugs for cancer therapy. But nowhere is a good explanation of why these combination of drugs are used. What is the basis of using such combinations?...

15 October 2021 6,310 1 View

I want to remove guanidine hydrochloride from cell lysate for proteomics analysis by dialysis.What is the ideal composition of the dialysis buffer?

The dialysis buffer composition for proteomics analysis of cell lysate to remove Gn-HCl by dialysis

04 March 2021 2,786 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

03 March 2021 3,964 2 View

For gelatin coating for culturing HUVEC.Should the gelatin be made in sterile water or PBS? Do I need to autoclave the gelatin soln after making it ?

I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?

01 March 2021 504 2 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

01 March 2021 7,979 3 View

I want to synthesize a GUV. Can you tell the lipid composition and ratio that I should use that will mimic the biological cell/endosomal membrane?

Can you please provide me the reference

18 November 2020 6,264 2 View

Which company offers cell lines in India at an economical price?

the cell lines should be economical and authentic. is there any Chinese Japanese Indian or any other company who ships cell lines to India at an economical price? I know about NCCS , anything...

15 July 2020 3,709 0 View

For how long should a mammalian cell line be kept in -80 degree C after transferring it from liq nitrogen to culture the cell in media?

for how long is it ideal to keep in -80 degree celsius after transferring from liq nitrogen to culture the cell

03 July 2020 10,085 1 View

From where can I get cisplatin resistant breast cancer cell line?

I do research in Kolkata, India?

07 October 2019 9,533 3 View

From where can I get radioressistant kidney cancer and breast cancer cell line?

The cell lines should be radiation resistant

07 October 2019 7,637 1 View

For determining IC50 with MTT what time point should I use?

Example of time points like 24hrs or 48 hrs or 72 hrs . The drug is Doxorubicin. Cell line used is breast cancer cell line MDA-MB-231

16 October 2018 8,844 3 View

I culture my cells in RPMI-1640, will it be harmful for the cell if i use opti-MEM for some some time for certain experiments?

In my lab cells are cultured in DMEM and for certain experiments like to see the intake by cells we use opti-mem media. But my new cell type is being cultured in RPMI-1640 with 1mM sodium pyruvate...

10 May 2018 2,391 3 View

What problem can arise in Western and Immunofluorescence if my primary antibody consists of only kappa light chains and not the whole antibody?

What type of problems can I face? For standardizing an experiment is whole antibody preferable over primary antibodies consisting only of kappa light chains? Why such problem arises?

01 February 2018 8,304 1 View

I want to check upregulation of receptors and other proteins in a tissue lysate. What method should I follow?

Please refer papers with detailed protocol that can be understood for biology students

08 December 2017 9,891 3 View

What is the detailed protocol to make long peptides using biotechnological approach?

Please provide the references. The peptides would be used for therapeutic purposes for humans

21 October 2017 7,017 3 View

What is the percentage of knockdown if I use HiPerFect transfecting agent on MDA MB231 cell line?

mainly for ERK1 silencing

24 August 2017 7,577 2 View