Hello all the members. I am getting negative values when I have taken the derivative of weight loss(%) w.r.t Temperature, what is this implies ?
Thank you
Hello Everyone, I am culturing few different kinds of adherent cells. However, I sometimes experience cells dying in the flask after passaging them in one flask whereas same cells suspension...
07 February 2024 4,354 0 View
I have a question regarding culturing fibroblast IMR90. I use DMEM+10%fbs+1% pen/strep for culturing them. I use 0.05% trypsin EDTA for trypsinization for 40 sec in the incubator and add DMEM +...
18 January 2024 958 1 View
20 December 2023 4,363 0 View
What should be the final conc of PI used?
11 October 2023 587 2 View
I want to know about the mechanism through which biological nitrogen fixation occurs in photosynthetic green microalgae apart from previously reported bacterial associations. Like in axenic lab...
11 August 2023 3,256 2 View
In our lab we have cell culture wares for adherent cell lines which are coated. Can I use the same flask for culturing suspension cell line (Jurkat)?
25 June 2023 4,412 2 View
Can I calculate the optical energy band gap from the absorbance of the UV-Vis spectrophotometer, or It can only be calculated from the reflectance? Thank you
27 March 2023 9,503 1 View
we have synthesized anticancer drugs which are toxic to cancer cells. but it is desirable that such drugs are non toxic to normal cells. pls provide me a list of normal cell lines where such...
13 March 2023 2,516 4 View
Answers as per your knowledge and experiences.
23 December 2022 8,918 19 View
Greetings all, I am working on developing a multipole analysis formalism for plasmonic metasurfaces that involves the extraction of volume current densities in A/m2 in order to calculate the...
25 September 2022 1,611 0 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I am working on polymerizing monomers similar to lactic acid, each containing one carboxylic acid and one alcohol group, through a polycondensation technique. Despite using vacuum conditions...
20 June 2024 2,966 4 View
I wish to know more on this. Any help or suggestion are highly appreciated. Is there any technique to find it out? Being new in this field.
19 June 2024 3,824 4 View
I use molecular sieve 13X and Iron nitrate powder Fe(NO 3)3 • 9H2O. and commercial TiO2. I need to prepare 8% Fe, 8% TiO2 containing with zeolite 13X. I want to use this method below: WET...
17 June 2024 4,153 2 View
1) Does accreditation or background of authors play a role in achieving a closer nutritional value to the established recommended dietary guidelines for weight loss?
14 June 2024 1,322 0 View
Help me to draw graph of Bioconcentration
10 June 2024 4,941 2 View
I am working with snake venom, specifically targeting toxins around the 15 kDa range. I need to remove higher molecular weight toxins (above 20 kDa). Would it be advisable to use a 20 kDa MWCO...
05 June 2024 691 1 View
Hi, there are 2 bands in my protein's MW. What could be the reason for shifting the band from the original band? My protein's molecular weight is 131 KDa and it is bound with a FAD cofactor. I...
28 May 2024 4,294 1 View
Greeting, I am using PCL and IPPDI to produce the Thermoplastic Polyurethane with different ratio. I would like to calculate the amount of PCL and PPDI that need to produce 0.2 and 0.4 ratio of...
23 May 2024 9,448 0 View