Alexis Cecilia Hruby

7 Questions 8 Answers 0 Followers

Questions related from Alexis Cecilia Hruby

Do milk fat globules prevent cell growth?

Hi everyone! I have had good luck asking questions before so I thought I should ask this. Has anyone else ever noticed that fat globules surrounding secretory tissue seem to prevent cells from...

17 August 2021 2,793 4 View

What is the difference between blocking buffer tbs vs. pbs and which should I use?

Hi everybody! I am doing western blots on bovine mammary epithelial cells to determine casein. We have two types of blocking buffer in the lab. PBS:...

02 February 2021 9,615 5 View

What types of tubes should I use for allantoin assay?

Hi everyone, I am doing an allantoin assay in urine and all the protocols seem pretty vague. I am currently using this website which seems to be the most helpful:...

08 January 2021 8,217 3 View

What is growing inside a frozen 10X Ripa buffer?

Hello all! I have a strange question today...I noticed that a few of my aliquotted 10x RIPA buffers that were in the -20 C had some kind of potential mold (white balls, some clusters, some with...

21 December 2020 7,593 3 View

What are these called?

Upon tissue plating for cell isolation, I start to see fibroblasts and after a while these 3D structures start to form. Are they called stroma? Someone in my building told me that she calls them...

01 December 2020 8,848 4 View

Plated Secretory Tissue from Udder--What type of cells are these?

Hello all! I plated some secretory tissue from the udder of a dairy cow. I am trying to isolate mammary epithelial cells. I found these cells on one of my plates, are these developing mammary...

29 October 2020 7,911 3 View

How do you write a proc nlim (SAS) code in R to solve for feed degradability? How do we write a conditional in R for lag time?

My advisor sent me the following code and told me to rewrite it in R. proc nlin; parms a=19 c=9 k=.08 lag=2; *a=soluble, c=undegradable, k=rate/h, lag=lag time; time=time-lag; if time

01 January 1970 6,383 3 View