Hello,
I have got the purified recombinant enzyme and were eluted in 50mM sodium phosphate buffer and stored it in 15% glycerol at -20oC. The substrate for the enzyme is farnesyl pyrophosphate (FPP), which I have obtained from SIGMA and it came as farnesyl pyrophosphate ammonium salt (Methanol:ammonia::7:3). For how long will it be stable if diluted in phosphate buffer and how should I proceed further for analysis?
Water solubility of farnesyl pyrophosphate at 25o ): 0.3693 mg/L
(According to http://www.chemspider.com/Chemical-Structure.393270.html)
It is very stable at pH 7.5-8.0 especially at low temperature. Any time you have some doubts you can analyse it by using 31P NMR. It will take 3-5 min and easily reveal orthophosphate accumulation.
Best wishes!
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