Hello,
Is there any alternative (cheaper) Bio-Rad AutoDG-compatible tips? The price for a pack of 20 boxes has become double in 2024.
Thanks
Hi, I am facing a temperature convergence issue in Silvaco Atlas. At the threshold voltage the temperature is not converging. I applied given models models consrh bgn FLDMOB HCTE MASETTI...
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In my ddPCR assay to check my specific edits the PCR amplicon length is 100 bp, and two probes are within the amplicon. Probe 1 (labelled with FAM) is specific to mutant and Probe 2 (labelled with...
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Need guidance and help in understanding and interpretation of some data regarding the components forces that we get from the dynamometer after the side milling (up and down) operation. After the...
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Hi, I am currently using Bio-Rad Automated Droplet generator. I am happy with the result. I get on average 19K to 21K droplets per reaction. However, I have also encountered 1 to 2 samples...
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I have designed a ddPCR assay to detect gene edits in my organism. The PCR amplicon length is 100 bp, and two probes are within the amplicon. Probe 1 (labelled with FAM) is specific to mutant and...
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Can I get brief explanation about this this theorem, which is applied in venturi meter ?
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I am working on an Abaqus User element and have 4 degrees of freedom(3 displacement and 1 user-defined). I am working on a single element example, where I want to define the initial condition of...
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I am currently planning on performing metabolic flux analysis in plants using isotopic sulfur. but I am stuck on how to replace the normal sulfur-containing nutrients in MS media ( such as...
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After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
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i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
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I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
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Hoy la animación se utiliza como una tecnología multimedia con gran potencial educativo, que va mucho más allá de sólo crear figuras, ya que puede promover una mejor comprensión en...
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I am currently working on validating land subsidence results obtained using SBAS InSAR. Could anyone suggest reliable sources or repositories where I can download a historical DEM from 2018?...
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Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
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Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
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Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
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