Hello,
Is there any alternative (cheaper) Bio-Rad AutoDG-compatible tips? The price for a pack of 20 boxes has become double in 2024.
Thanks
I am making microfluidic devices made of polyurethane. The problem I am facing is that I am unable to attach these devices to glass slide even after plasma treatment (air plasma). Some of the...
28 January 2021 5,194 2 View
01 July 2020 9,998 2 View
Dear all, I am working on a magnetic beads based RNA extraction project. Presently, I managed to come up with a lysis to extract RNA from blood samples. However, my PCR results show inhibitory...
06 April 2020 9,953 3 View
I am doing an experiment with colloidal solution of nanoparticles and would like to measure the Brownian motion velocity of these particles. Can I do it experimentally?
09 March 2020 7,416 12 View
I am researching how different culture and expansion conditions contribute to a T cell's infiltration of a solid tumor. Was wondering what the best surface / intracellular receptors or ligands are...
26 June 2019 5,072 0 View
Consider an initially non-hydrated gamma-alumina surface. I pass water over the surface hoping it to dissociatively adsorb on the surface giving me hydroxyl radicals adsorbed on alumina surface....
31 March 2019 8,615 2 View
I am working on Gaucher"s disease, in this connection I would like to isolate GCase enzyme from rat brain. so please share the protocol if available. This isolation process should not include HPLC.
27 March 2019 2,591 1 View
Dear Researchers, How to define a collimating lens in TracePro Software? Thanks and Regards, Dhanu Chettri
05 September 2018 4,002 2 View
Me and a group of first year undergrads from my college are working on building a horn-antenna for radio astronomy similar to this...
16 April 2018 8,040 3 View
Dear Researchers, What is the effective mass of electron and hole of InxGa1-xN and does the effective mass depends on the indium mole fraction x? and Does it also depends on the thickness of the...
09 April 2018 8,418 2 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...
02 March 2021 8,481 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View