To culture the U-87-MG cell line, various media formulations are available online. These include:
a. Essential Minimum Eagle Medium (EMEM) supplemented with 10% fetal bovine serum (FBS), 100 U/mL penicillin, and 100 µg/mL streptomycin.
b. Dulbecco's Modified Eagle Medium (DMEM) with 10% FBS.
c. F-12 medium.
When seeking the best-suited medium for culturing the U-87-MG cell line, options such as EMEM supplemented with FBS and antibiotics, DMEM with FBS, and F-12 medium are commonly found. But I am not clear which media to use to ensure the best growing conditions among these?
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